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KMID : 1161519970010020341
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Animal Cells and Systems
1997 Volume.1 No. 2 p.341 ~ p.344
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Receptor-mediated transport of vitellogenin during oogenesis of a polychaete, pseudopotomilla occelata
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Lee Bong-Kyung
Nam Hyun-Jung
Lee Yang-Rim
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Abstract
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Receptor?mediated endocytosis has been suggested for a stage?specific transport mechanism of vitellogenin into the oocytes of a sabellid polychaete, Pseudopotamilla occelata. Membrane proteins of oocytes of three size classes, including small (30?70 μm in diameter), intermediate (70?140 μm in diameter) and large (180?200 μm in diameter), showed a stage?specific variation. Coelomic fluid proteins (CP), assumed to be vitellogenin, consists of several proteins, which showed quite a different pattern from that of yolk proteins. Incorporation of 125I?CP into the oocytes of the intermediate size class almost linearly increases with time, showing a contrast to the pattern of the large size class, in which the incorporation is low and approaches a plateau, suggesting the vitellogenin transports by a regulated process only in the intermediate size class. Vitellogenin receptor proteins were identified to be 60 kDa and 68 kDa only in the intermediate size class by a ligand blotting test.
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KEYWORD
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Receptor proteins, Vitellogenin, Oocytes, Polychaete
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